Figure 3.

Photomicrographs showing representative immunohistochemistry images of phosphorylation of ERK (p-ERK) protein in the dorsal horn from spinal cord sections from Shank2 KO (Shank2 −/−) or WT (Shank2 +/+) mice that were intrathecally treated with saline or NMDA. In WT mice, (b) NMDA treatment increased p-ERK expression in the superficial dorsal horn (SDH) as compared to (a) saline treatment. (c) This NMDA-induced increase in p-ERK expression is suppressed in Shank2 KO mice. The lower boxes (outlined in white) are magnified images of the superficial dorsal horn from each image. Scale bar = 200 µm.