Figure 7.

Overexpression of SOCS3 reversed the increase of TLR4 expression. (a) Western blots and bar graph showed the mean density relative to β-actin for TLR4 receptors from BC rats was significantly increased when compared with controls (CON; *p < 0.05; n = 4 for each group, student’s t-test). (b) After LV-SOCS3 treatment, the relative TLR4 receptors density was decreased when compared with LV-NC group (*p < 0.05; n = 4 for each group, student’s t-test). (c) Co-expression of SOCS3 with TLR4 receptors in tibia innervating DRG neurons. L5 DRG cells innervating the tibia were labeled with DiI in red (top left, arrows). SOCS3 positive cells are shown in blue (top middle). TLR4 receptors positive cells are shown in green (top right). Bar in the top left of figure (c) equals 50 µm.