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Retrogradely labeling of RVM neurons by injection of FluoroGold into the substantia nigra pars compacta (SNpc). (a) The injection site of FluoroGold in the right side and left side of SNpc. Green labelings are FG. Red labelings were TH-positive staining. (b) 5-HT immunostaining in the RVM showed that 5-HT neurons were not labeled by FG.
