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. Author manuscript; available in PMC: 2017 Feb 10.
Published in final edited form as: Biochemistry. 2016 Aug 4;55(32):4445–4456. doi: 10.1021/acs.biochem.6b00285

Figure 4.

Figure 4

Measuring microsecond-to-millisecond motions using relaxation dispersion in the rotating frame. Secondary structure (left) and representative RD profiles for (A) ES1 and (B) ES2 in wtTAR35,37 (middle) and ΔC24-wtTAR (right). RD profiles show the dependence of R2 + Rex on spin lock power (ωeff/2π) and offset (Ω/2π) with global fits (—) and individual fits (–) to the two-state Laguerre equation (eq 2).82 Error bars represent experimental uncertainty [one standard deviation (see Methods)].