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(A) SDS-PAGE of induced (+) and non-induced (−) cultures for clones A and B. The position of the induced mature rAra h 1 is indicated with an arrow. (B) SDS-PAGE of the soluble and insoluble fractions of induced cells (A and B clones) following lysis. “sup” is the soluble supernatant. “pell” is the insoluble cellular debris. (C) Western blot analysis with anti-Ara h 1 antibody of the same samples shown in B.
