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. 2016 Mar 31;6(4):57. doi: 10.3390/nano6040057

Figure 4.

Figure 4

INTEGRA® nanocomposite material is a suitable for culture of L-ASCs and B-ASCs. L-ASCs (a) and B-ASCs (b) cultured on INTEGRA® have similar viability to stem cells cultured on TCP as demonstrated by 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay measured at Absorbance (ABS) 450nm. L-ASCs (c,d) and B-ASCs (e,f) cultured on INTEGRA® maintained the fibroblast-like morphology. Representative images of cytoskeleton fibers (F-Actin, Tubulin), focal adhesion spot (Vinculin) and nuclei (DAPI: diamidino-2-phenylindole) were acquired with fluorescence microscopy Eclipse-TE2000-S, Nikon, using the F-ViewII FireWireTM camera (Soft Imaging System, Olympus).