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Effect of the starting HSA concentration (mg/mL) on the (a) HSA-NP size (mean ± SD, n = 10), represented as columns, and polydispersity index, represented as line; (b) zeta potential of HSA-NPs (mean ± SD, n = 10), represented as columns, prepared with a chloroform concentration of 3% v/v of starting HSA solution, 12 homogenization cycles and 20,000 psi homogenization pressure (*** p < 0.0001; ns = not significant).
