Table 3.
Identification of up- and don-represented proteins of the roots of cowpea genotype CE-31 after being challenged with M. incognita (Race 3) in relation to non-infected control plants.
| Spot *No. | Accession No. (NCBI) | Protein Identification | Organism | pI/MW (kDa) | Score | Sequence Covered (%) | Sequences | |
|---|---|---|---|---|---|---|---|---|
| Experimental | Theoretical | |||||||
| Functional category: Disease/defense | ||||||||
| 2↑ | gi/297493 | ACC synthase | Vigna radiata | 4.89/39.678 | 5.40/43.768 | 79 | 7.98 | YFDGWK |
| VHIVYSLSK | ||||||||
| VGTIYSYNDSVVTTAR | ||||||||
| 3↑ | gi/86197901 | ACC oxidase | Vigna radiate | 5.05/38.056 | 5.86/40.236 | 111 | 12.30 | GAAMEMIK |
| EMVANK | ||||||||
| VSNYPPCPTPDLIK | ||||||||
| DDQWIDVPPMR | ||||||||
| 11↑ | gi/42795352 | Ascorbate peroxidase | Vigna unguiculata | 6.62/28238 | 6.67/31.746 | 108 | 11.84 | NCAPLMLR |
| EIVALSGGHTLGR | ||||||||
| SGFDGPWTEDPLK | ||||||||
| 15↑ | gi/13274148 | CuZn-superoxide dismutase | Populus tremula | 6.76/23.114 | 5.87/29.203 | 142 | 14.74 | AVAVLK |
| LTHGAPEDEIR | ||||||||
| GGHELSSTTGNAGGR | ||||||||
| 16↑ | gi/123539 | 17.5 kDa HSP | Glycine max | 5.87/22.268 | 6.12/17.412 | 177 | 18.18 | DFHVPTSSVSAENSAFVSTR |
| VLQISGER | ||||||||
| 17↑ | gi/130829 | PR-1 | Phaseolus vulgaris | 4.44/16.126 | 4.83/16.528 | 168 | 18.58 | ALPDSFK |
| ISFVEDGETK | ||||||||
| LSDGPNGGSLIK | ||||||||
| 18↑ | gi/4850337 | PR-3 (Chitinase) | Vigna unguiculata | 4.55/15.971 | 4.75/16.265 | 122 | 14.28 | ISFLEDGETK |
| LSDGSNGGSVVK | ||||||||
| 19↑ | gi/ 130835 | PR-2 (β-1,3-glucanase) | Phaseolus vulgaris | 5.11/16.352 | 4.85/16.402 | 86 | 10.96 | ISIDSK |
| GDAPPNEDELK | ||||||||
| Functional category: Secondary metabolism | ||||||||
| 6↑ | gi/27530706 | Chalcone-flavone isomerase | Lotus japonicus | 6.41/35666 | 5.94 /36.987 | 97 | 11.11 | SYFLGGAGER |
| STGTYGEAEAAAIGK | ||||||||
| Functional category: Metabolism | ||||||||
| 21↑ | gi/ 20138591 | Leghemoglobin | Vigna unguiculata | 6.00/14.722 | 5.68/15.341 | 225 | 31.03 | ADIPK |
| NLFSFLANGVDATNPK | ||||||||
| ASGGVVADAALGAVHSQK | ||||||||
| EAVGDK | ||||||||
| 22↑ | gi/ 26245403 | Nucleoside diphosphate Kinase | Glycine max | 6.89/15.964 | 6.30/16.254 | 183 | 21.08 | PDGVQSGLIGEIISR |
| IIGATNPAQSEPGTIR | ||||||||
| Functional category: Protein destination and storage | ||||||||
| 1↓ | gi/ 4589396 | Asparaginyl endopeptidase | Vigna mungo | 4.38/49.316 | 5.14/52.982 | 77 | 6.83 | FPIIFVVANLITLVSGGR |
| NSLVPPSK | ||||||||
| APLGSSR | ||||||||
| 4↑ | gi/ 1223922 | Cysteinyl endopeptidase | Vigna radiata | 5.10/35.289 | 5.44/37.332 | 64 | 6.35 | LLWVVLSLSLVLGVANSFDFHEK |
| Functional category: Unknown/Predicted/Uncharacterized | ||||||||
| 7↑ | gi/297849580 | Predicted protein | Arabidopsis lyrata | 5.03/25.370 | 6.22/33.633 | 119 | 12.58 | EVETLPEEAFEEEEDK |
| EILENHGGEER | ||||||||
| IMDEAVNASR | ||||||||
| 9↑ | gi/ 297824991 | Predicted protein | Arabidopsis lyrata | 5.26/25.967 | 7.97/39.819 | 58 | 5.54 | QVVDETEPK |
| VYGSIEEHYYR | ||||||||
| 10↓ | gi/2970051 | ARG 10 | Vigna radiata | 5.33/30.326 | 5.62/25.480 | 133 | 14.34 | DEIFCLFEGALDNLGSLR |
| VVCHLSGSFAFIVFDK | ||||||||
| 12↑ | gi/416640 | Auxin induced protein | Vigna radiata | 4.81/19.354 | 4.65/21.345 | 104 | 11.34 | EGLGLEITELR |
| GYSDLAFALEK | ||||||||
* Arrows indicate up- (↑) and down-represented (↓) proteins. Functional categories are according to Bevan et al. [18].