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. 2013 Oct 11;1(3):180–218. doi: 10.3390/proteomes1030180

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Flowchart of the most currently used techniques in view of a comparative and quantitative proteomics approach using a protein-based approach (panel A) or a peptide-based approach (panel B). The proteomic analysis is made up of four steps: (i) sample conditioning (not illustrated); (ii) sample preparation; (iii) separation; and (iv) quantification and identification of the proteins. The separation can be performed on proteins or peptides, by electrophoresis or chromatography. The quantification is possible either in-gel or off-gel, whereas the identification is always performed by MS. MS, mass spectrometry; HPLC: high performance liquid chromatography; IEF: isoelectric focusing; PAGE: polyacrylamide gel electrophoresis; PMF: peptide mass fingerprint; PFF: peptide fragmentation fingerprint.