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. 2013 Oct 11;1(3):180–218. doi: 10.3390/proteomes1030180

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Comparison of the proteins extracted with Triton X-100 from bovine brain capillary endothelial cells showing limited (Lim. BBB) (A) or re-induced (Re-ind. BBB) BBB functionalities (B). Digital image obtained after 2D-PAGE of the proteins separated according to their pI and MW. The gel was silver nitrate stained. The numbering corresponds to the enriched protein in each condition. Each spot was identified by peptide mass fingerprinting (PMF) and/or peptide fragmentation fingerprinting (PFF) on a Proteineer ^TM workstation (adapted with permission from [45]).