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Differences between label free quantification (A) and quantification by means of stable-isotope labelling (B). The label free quantification consists of two analyses carried out independently before their comparison. The quantification by means of stable-isotope labelling allows the direct comparison of isotope-labelled peptide pairs. LC-MS/MS, liquid chromatography coupled to tandem MS. (Adapted from [69], Creative Common Attribution License CC-BY).
