Table 2.
A representative sample among the proteins identified during 2D-PAGE. The proteins were extracted with Triton X-100 from bovine brain capillary endothelial cells with limited (Lim. BBB) or re-induced (Re-ind. BBB) BBB functionalities. The Table illustrates the presence of one spot for one protein, multiple proteins for one spot and/or multiple spots for one protein. The identifications were done by PMF and PFF after MALDI-TOF/TOF mass spectrometry. Proteins over-abundant in Lim. BBB are highlighted in grey. The data were reproduced with permission from [45].
| Spot number | Protein Name | Swiss-Prot Accession | Theoretical | Experimental | PMF Mascot Score c | Sequence Coverage (%) | Matched/ Unmatched peptides | Identification by MS or MS/MS | Number of fragmented peptides | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MW (kDa) a | pI b | MW (kDa) a | pI b | |||||||||
| One spot for one protein | 45 | Serine-threonine kinase receptor-associated protein | STRAP_BOVIN | 38.4 | 4.99 | 40.5 | 4.90 | 242 | 67 | 21/25 | MS | |
| 47 | Inorganic pyrophosphatase | IPYR_BOVIN | 32.8 | 5.27 | 39.7 | 5.09 | 168 | 42 | 12/14 | MS | ||
| 58 | Phosphatidylinositol transfer protein alpha isoform | PIPNA_BOVIN | 31.8 | 6.12 | 34.8 | 5.54 | 73 | 28 | 8/15 | MS | ||
| 72 | 6-phosphogluconolactonase | 6PGL_BOVIN | 27.6 | 5.57 | 29.9 | 5.44 | 129 | 40 | 10/19 | MS & MS/MS | 1 | |
| 78 | Apolipoprotein A-I precursor (Apo-AI) | APOA1_BOVIN | 30.2 | 5.71 | 26.2 | 5.52 | 366 | 68 | 25/16 | MS | ||
| 79 | Sorcin | SORCN_HUMAN | 21.7 | 5.32 | 24.1 | 4.98 | 153 | 48 | 15/28 | MS | ||
| One spot for several proteins | 36 | Actin, cytoplasmic 1 (Beta-actin) | ACTB_BOVIN | 41.7 | 5.29 | 44.2 | 5.52 | 94 | 44 | 13/50 | MS & MS/MS | 1 |
| Succinyl-CoA ligase [GDP-forming] beta-chain, mitochondrial [Precursor] | SUCB2_BOVIN | 46.7 | 7.51 | 44.2 | 5.52 | 112 | 28 | 14/49 | MS/MS | 1 | ||
| 37 | Actin, cytoplasmic 1 (Beta-actin) | ACTB_BOVIN | 41.7 | 5.29 | 44.2 | 5.66 | 80 | 44 | 14/91 | MS | ||
| Leukocyte elastase inhibitor | ILEU_BOVIN | 42.2 | 5.70 | 44.2 | 5.66 | 134 | 49 | 22/83 | MS | |||
| Succinyl-CoA ligase [ADP-forming] beta-chain, mitochondrial [Precursor] | SUCB1_BOVIN | 50.0 | 6.73 | 44.2 | 5.66 | 100 | 44 | 20/85 | MS | |||
| Several spots for one protein | 67 | Chloride intracellular channel protein 4 | CLIC4_BOVIN | 28.7 | 5.6 | 30.9 | 5.42 | 159 | 68 | 19/47 | MS | |
| 70 | Chloride intracellular channel protein 4 | CLIC4_BOVIN | 28.7 | 5.6 | 30.2 | 5.69 | 288 | 81 | 22/14 | MS | ||
| 13 | Vimentin | VIME_BOVIN | 53.7 | 5.06 | 57.0 | 4.91 | 233 | 56 | 23/16 | MS & MS/MS | 1 | |
| 16 | Vimentin | VIME_BOVIN | 53.7 | 5.06 | 52.3 | 4.82 | 373 | 78 | 45/48 | MS & MS/MS | 3 | |
| 39 | Vimentin | VIME_BOVIN | 53.7 | 5.06 | 42.5 | 4.55 | 296 | 69 | 37/39 | MS & MS/MS | 1 | |
| 40 | Vimentin | VIME_BOVIN | 53.7 | 5.06 | 41.7 | 4.54 | 228 | 57 | 29/37 | MS & MS/MS | 1 | |
| 41 | Vimentin | VIME_BOVIN | 53.7 | 5.06 | 43.1 | 4.53 | 118 | 33 | 10/11 | MS | ||
| 64 | Vimentin (Fragment) | VIME_BOVIN | 17.2 | 9.92 | 32.2 | 5.16 | 89 | 27 | 11/19 | MS | ||
| 65 | Vimentin | VIME_BOVIN | 53.7 | 5.06 | 31.6 | 5.38 | 265 | 42 | 34/43 | MS & MS/MS | 4 | |
| 66 | Vimentin | VIME_BOVIN | 53.7 | 5.06 | 35.3 | 5.16 | 205 | 42 | 21/16 | MS | ||
| 76 | Vimentin | VIME_BOVIN | 53.7 | 5.06 | 26.7 | 4.50 | 110 | 32 | 12/20 | MS & MS/MS | 1 | |
a Molecular weight; b Isoelectric point; c Score correspond to the measures of certainty (p-values <0.05).