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. 2014 Jan 22;2(1):18–52. doi: 10.3390/proteomes2010018

Table 3.

Advantages and disadvantages of chromatographic and capillary electrophoretic techniques in proteomics. CE, capillary electrophoresis; IACE, immunoaffinity CE.

Method Advantages Disadvantages
LC-MS

  • -High sensitivity

  • -High specificity

  • -High resolution

  • -Very expensive equipment and columns

  • -Relatively high volumes of sample injected (µL)

  • -Very high expertise needed
CE-MS

  • -Less expensive than LC-MS

  • -Very small volumes of samples injected (nL)

  • -Lower expertise needed compared to LC-MS

  • -Basic and hydrophilic peptides with low molecular masses easier to be detected than in LC-MS

  • -Fast separation

  • -Limited loading capacity

  • -Unavailability of an integrated system as a marketed solution
IACE

  • -Enrichment and quantification of ultra-low abundance analytes in complex biological matrices

  • -Combines high-resolving power of CE and on-line coupling of high selective antibody-capture agents.

  • -Very fast separation

  • -Separation and quantification of intact substances and their respective modified counterparts.

  • -Potential for measuring single-cell components

  • -Miniaturization

  • -Low cost

 Apparently not one of the disadvantages indicated for other techniques is observed in this capillary electrophoretic approach.