Appendix Table A1.
Induction of significantly changed proteins in radicles of seeds derived from aluminum-treated tomato plants z.
| Protein accessions y | Protein name x | log2 fold w (Treated/control) | Ratio v Treated/control) |
|---|---|---|---|
| Solyc03g019820.2.1 | Aquaporin | −1.88 | 0.27 |
| Solyc06g034040.1.1 | Oleosin | −1.73 | 0.30 |
| Solyc06g072130.2.1 | Aquaporin | −1.64 | 0.32 |
| Solyc02g086490.2.1 | Oleosin | −1.61 | 0.33 |
| Solyc02g084840.2.1 | Dehydrin DHN1 | −1.53 | 0.35 |
| Solyc03g112440.1.1 | Oleosin | −1.52 | 0.35 |
| Solyc06g072670.2.1 | Short-chain dehydrogenase/reductase SDR | −1.47 | 0.36 |
| Solyc06g053740.2.1 | Ubiquitin carboxyl-terminal hydrolase | −1.42 | 0.37 |
| Solyc01g109920.2.1 | Dehydrin | −1.35 | 0.39 |
| Solyc06g065050.1.1 | Transmembrane protein 205 | −1.33 | 0.40 |
| Solyc02g077240.2.1 | Pyruvate decarboxylase | −1.30 | 0.41 |
| Solyc12g010920.1.1 | Oleosin | −1.28 | 0.41 |
| Solyc09g082330.1.1 | 7S vicilin | −1.24 | 0.42 |
| Solyc12g096930.1.1 | Caleosin | −1.18 | 0.44 |
| Solyc10g008040.2.1 | Seed biotin-containing protein SBP65 | −1.17 | 0.44 |
| Solyc11g067250.1.1 | Poly (AHRD V1 ***- B9SCR8_RICCO) | −1.11 | 0.46 |
| Solyc02g085590.2.1 | Vicilin | −1.10 | 0.47 |
| Solyc11g072380.1.1 | Vicilin-like protein | −1.09 | 0.47 |
| Solyc06g075270.2.1 | Convicilin | −1.07 | 0.48 |
| Solyc01g100390.2.1 | Pyrophosphate-energized proton pump | −1.06 | 0.48 |
| Solyc06g009210.2.1 | Ribosomal protein L19 | −1.03 | 0.49 |
| Solyc09g025210.2.1 | Legumin 11S-globulin | −1.03 | 0.49 |
| Solyc05g053140.2.1 | 26S proteasome non-ATPase regulatory subunit 13 | −1.02 | 0.49 |
| Solyc10g076510.1.1 | Pyruvate decarboxylase | −1.01 | 0.50 |
| Solyc05g053120.1.1 | Glucosyltransferase | −0.99 | 0.50 |
| Solyc08g014000.2.1 | Lipoxygenase | −0.98 | 0.51 |
| Solyc08g078850.2.1 | L-lactate dehydrogenase | −0.95 | 0.52 |
| Solyc01g009660.1.1 | Low-temperature-induced 65 kDa protein | −0.94 | 0.52 |
| Solyc01g098850.2.1 | Short-chain dehydrogenase/reductase family protein | −0.94 | 0.52 |
| Solyc06g076640.2.1 | Tubulin beta chain | −0.94 | 0.52 |
| Solyc03g116590.2.1 | Embryo-specific 3 | −0.94 | 0.52 |
| Solyc06g059740.2.1 | Alcohol dehydrogenase 2 | −0.93 | 0.53 |
| Solyc11g042800.1.1 | Late embryogenesis abundant protein | −0.93 | 0.53 |
| Solyc03g025810.2.1 | Low-temperature-induced 65 kDa protein | −0.92 | 0.53 |
| Solyc03g083970.2.1 | IQ calmodulin-binding motif family protein | −0.91 | 0.53 |
| Solyc08g013860.2.1 | NAD-dependent malic enzyme 2 | −0.90 | 0.53 |
| Solyc10g078770.1.1 | Seed maturation protein LEA 4 | −0.90 | 0.53 |
| Solyc09g090150.2.1 | Legumin 11S-globulin | −0.89 | 0.54 |
| Solyc03g112590.2.1 | Cell division protease ftsH homolog | −0.89 | 0.54 |
| Solyc04g064710.2.1 | Alcohol dehydrogenase 2 | −0.89 | 0.54 |
| Solyc00g297330.1.1 | Unknown Protein | −0.88 | 0.54 |
| Solyc07g053360.2.1 | Seed biotin-containing protein SBP65 | −0.87 | 0.55 |
| Solyc08g080480.2.1 | Unknown Protein | −0.87 | 0.55 |
| Solyc06g074750.1.1 | Histone H2B | −0.86 | 0.55 |
| Solyc12g098940.1.1 | Ubiquitin | −0.85 | 0.55 |
| Solyc07g032740.2.1 | Aspartate aminotransferase | −0.85 | 0.56 |
| Solyc01g007940.2.1 | Alanine aminotransferase 2 | −0.83 | 0.56 |
| Solyc09g065470.2.1 | Vicilin | −0.83 | 0.56 |
| Solyc09g015070.2.1 | Reductase 1 | −0.83 | 0.56 |
| Solyc12g014380.1.1 | Glucose-6-phosphate isomerase 1 | −0.81 | 0.57 |
| Solyc07g005390.2.1 | Aldehyde dehydrogenase | −0.81 | 0.57 |
| Solyc09g082340.2.1 | Vicilin-like protein | −0.80 | 0.57 |
| Solyc01g107910.2.1 | Caffeoyl CoA 3-O-methyltransferase | 0.80 | 1.74 |
| Solyc00g009020.2.1 | Mitochondrial ATP synthase | 0.80 | 1.74 |
| Solyc06g063220.2.1 | ATP synthase subunit epsilon mitochondrial | 0.80 | 1.74 |
| Solyc02g082090.2.1 | Peroxidase | 0.80 | 1.74 |
| Solyc01g102830.2.1 | Unknown Protein | 0.81 | 1.75 |
| Solyc00g147570.2.1 | Gelsolin | 0.81 | 1.75 |
| Solyc01g080510.2.1 | Os05g0406000 protein | 0.81 | 1.75 |
| Solyc08g068220.2.1 | 50S ribosomal protein L27 | 0.81 | 1.75 |
| Solyc03g078000.2.1 | High-affinity fructose transporter ght6 | 0.81 | 1.76 |
| Solyc03g096840.2.1 | Seed specific protein Bn15D1B | 0.82 | 1.76 |
| Solyc06g075810.2.1 | NADH dehydrogenase | 0.82 | 1.77 |
| Solyc06g007630.1.1 | Ferredoxin | 0.82 | 1.77 |
| Solyc05g007800.2.1 | Negatively light-regulated protein | 0.83 | 1.77 |
| Solyc11g072450.1.1 | Mitochondrial F0 ATP synthase D chain | 0.83 | 1.78 |
| Solyc10g078450.1.1 | U6 snRNA-associated Sm-like protein LSm6 | 0.83 | 1.78 |
| Solyc10g011760.2.1 | Aldose 1-epimerase family protein | 0.84 | 1.79 |
| Solyc11g066390.1.1 | Superoxide dismutase | 0.84 | 1.79 |
| Solyc03g078670.1.1 | Unknown Protein | 0.85 | 1.80 |
| Solyc05g053960.2.1 | Cysteine-rich extensin-like protein-2 | 0.85 | 1.81 |
| Solyc03g097360.2.1 | BolA-like | 0.85 | 1.81 |
| Solyc05g056020.2.1 | V-type proton ATPase subunit G 2 | 0.86 | 1.81 |
| Solyc07g063630.2.1 | Vesicle-associated membrane family protein | 0.86 | 1.82 |
| Solyc04g082590.2.1 | Canopy homolog 2 | 0.86 | 1.82 |
| Solyc02g079750.2.1 | Flavoprotein wrbA | 0.87 | 1.82 |
| Solyc02g078540.2.1 | Unknown Protein | 0.87 | 1.82 |
| Solyc11g065270.1.1 | CHCH domain containing protein | 0.87 | 1.83 |
| Solyc01g007670.2.1 | 30S ribosomal protein S7 chloroplastic | 0.87 | 1.83 |
| Solyc07g021500.1.1 | Unknown Protein | 0.87 | 1.83 |
| Solyc06g083820.2.1 | 60 ribosomal protein L14 | 0.88 | 1.84 |
| Solyc00g072400.2.1 | Peroxidase 1 | 0.88 | 1.84 |
| Solyc08g006900.2.1 | Ribosomal protein L32 | 0.88 | 1.84 |
| Solyc10g007350.2.1 | Multiprotein bridging factor 1 | 0.88 | 1.84 |
| Solyc07g055250.2.1 | Cell wall-associated hydrolase | 0.89 | 1.85 |
| Solyc08g075830.2.1 | Peroxidase 27 | 0.89 | 1.85 |
| Solyc05g041610.1.1 | Caffeoyl-CoA O-methyltransferase | 0.89 | 1.86 |
| Solyc07g008350.2.1 | Porin/voltage-dependent anion-selective channel protein | 0.89 | 1.86 |
| Solyc11g011340.1.1 | Alcohol dehydrogenase | 0.90 | 1.86 |
| Solyc12g094700.1.1 | Cathepsin B-like cysteine proteinase | 0.90 | 1.87 |
| Solyc01g049960.2.1 | Unknown Protein | 0.92 | 1.89 |
| Solyc03g114970.2.1 | Nitrilase associated protein-like | 0.92 | 1.89 |
| Solyc09g082710.2.1 | Histone H2A | 0.92 | 1.89 |
| Solyc08g016420.2.1 | Prefoldin subunit 6 | 0.92 | 1.90 |
| Solyc03g025850.2.1 | Remorin 1 | 0.93 | 1.91 |
| Solyc01g103220.2.1 | Cytochrome c | 0.94 | 1.92 |
| Solyc07g065640.2.1 | RPM1 interacting protein 4 transcript 2 | 0.94 | 1.92 |
| Solyc05g056290.2.1 | Acetyl-CoA carboxylase biotin carboxyl carrier protein | 0.95 | 1.93 |
| Solyc04g049330.2.1 | V-type proton ATPase subunit G 1 | 0.96 | 1.94 |
| Solyc01g091130.2.1 | Nitroreductase | 0.96 | 1.95 |
| Solyc01g095150.2.1 | Late embryogenesis abundant protein | 0.97 | 1.96 |
| Solyc07g005240.2.1 | FAD-dependent oxidoreductase family protein | 0.97 | 1.97 |
| Solyc01g090360.2.1 | Non-specific lipid-transfer protein | 0.98 | 1.97 |
| Solyc01g095050.2.1 | Negatively light-regulated protein | 0.98 | 1.98 |
| Solyc04g082010.1.1 | Plastocyanin | 1.00 | 1.99 |
| Solyc11g008990.1.1 | Phage shock protein A PspA | 1.00 | 2.00 |
| Solyc04g007750.2.1 | Major latex-like protein | 1.01 | 2.01 |
| Solyc03g113730.2.1 | B12D protein | 1.03 | 2.04 |
| Solyc08g013930.2.1 | Peroxidase family protein | 1.04 | 2.05 |
| Solyc01g088140.2.1 | Unknown Protein | 1.04 | 2.05 |
| Solyc02g085230.2.1 | Nucleolar protein 6 | 1.04 | 2.06 |
| Solyc06g036380.1.1 | Ulp1 protease family C-terminal catalytic domain containing protein | 1.04 | 2.06 |
| Solyc12g019040.1.1 | Exostosin family protein | 1.04 | 2.06 |
| Solyc03g116060.2.1 | Gibberellin-regulated protein | 1.04 | 2.06 |
| Solyc06g054520.1.1 | 3-hydroxyisobutyryl-CoA hydrolase | 1.05 | 2.07 |
| Solyc02g043900.1.1 | Unknown Protein | 1.06 | 2.08 |
| Solyc07g041490.1.1 | Stress responsive alpha-beta barrel domain protein | 1.07 | 2.09 |
| Solyc04g071580.2.1 | Unknown Protein | 1.08 | 2.12 |
| Solyc08g008330.2.1 | Unknown Protein | 1.09 | 2.13 |
| Solyc09g074890.1.1 | Rapid alkalinization factor 1 | 1.11 | 2.15 |
| Solyc04g024840.2.1 | GDSL esterase/lipase 1 | 1.11 | 2.16 |
| Solyc04g074900.2.1 | 40S ribosomal protein S21 | 1.12 | 2.17 |
| Solyc06g054250.2.1 | 5&apos-nucleotidase surE | 1.14 | 2.21 |
| Solyc02g092270.2.1 | NADH dehydrogenase | 1.14 | 2.21 |
| Solyc12g008950.1.1 | At1g17490/F1L3_4 | 1.15 | 2.22 |
| Solyc10g076240.1.1 | Peroxidase 1 | 1.21 | 2.31 |
| Solyc03g113580.1.1 | Germin-like protein | 1.26 | 2.40 |
| Solyc03g118110.2.1 | Succinate dehydrogenase assembly factor 2, mitochondrial | 1.30 | 2.45 |
| Solyc07g054960.1.1 | Myb-related transcription factor | 1.35 | 2.55 |
| Solyc10g005660.2.1 | COP9 signalosome subunit 6 | 1.35 | 2.55 |
| Solyc11g010160.1.1 | Cc-nbs-lrr, resistance protein | 1.35 | 2.55 |
| Solyc06g062770.2.1 | At1g17490/F1L3_4 | 1.36 | 2.56 |
| Solyc03g117810.2.1 | Phosphate import ATP-binding protein pstB 1 | 1.36 | 2.57 |
| Solyc11g066270.1.1 | Xyloglucan endotransglucosylase/hydrolase 9 | 1.37 | 2.59 |
| Solyc05g007090.2.1 | Zinc knuckle | 1.37 | 2.59 |
| Solyc01g107990.2.1 | MAP protein kinase-like protein | 1.42 | 2.68 |
| Solyc00g015000.1.1 | DNA (Cytosine-5-)-methyltransferase 3 | 1.55 | 2.93 |
| Solyc02g093230.2.1 | Caffeoyl-CoA O-methyltransferase | 1.55 | 2.93 |
| Solyc04g028490.1.1 | Ulp1 protease family C-terminal catalytic domain containing protein | 1.55 | 2.93 |
z Tomato proteins identified as significantly induced or repressed in tomato radicles from seeds germinated in 50 µM AlK (SO4)2 in 50 mM Homopipes buffer (pH, 4.5) (treated) and those in the buffer only (control). Tomato seeds were harvested from plants grown in a hydroponic solution supplemented with 50 µM AlK (SO4)2. y Protein accession number is from the ITAG Protein database (release 2.3 on 26 April 2011; Sol Genomics Network, Boyce Thompson Institute, Ithaca, NY, USA). X protein name annotated in ITAG2.3database. w The log2 ratio of each protein between treated and control samples measured by the intensity of its constituent peptides. All the listed proteins have passed the t test [general linear model (GLM)] with false discovery rate (FDR) corrections (p ≤ 0.05), and with a log2 fold change greater than 0.80 (±) which equals to two standard deviations of the near-normal distribution of log2 fold for all proteins identified in the experiment. Statistical analyses were performed using SAS (Version 9.3; SAS Institute Inc. Cary, NC, USA). V The ratio of protein abundance between treated and control samples, which is antilogarithm of the log2 ratio.