Table 1.
Differentially expressed spots post-irradiation identified in the study.
| Protein name | Name in database | SwissProt ID | Number of spots 1 | Spot number 2 | Irradiation 3,4 | Dose 3,5 | ||
|---|---|---|---|---|---|---|---|---|
| Max fold change (p < 0.05) | p-value | Max fold change (p < 0.05) | p-value | |||||
| Actin, beta | ACTB | ACTB_MOUSE | 1 | 1668 | = | / | −1.2 | 8.5 10−3 |
| Afamin | AFM | AFAM_MOUSE | 3 | 425, 438, 468 | +1.2 | 5.4 10−4 | +1.2 | 5.3 10−3 |
| Alpha-1-acid glycoprotein 1 (orosomucoid 1) | ORM1 | A1AG1_MOUSE | 1 | 919 | −1.2 | 3.7 10−3 | = | / |
| Alpha-1-antitrypsin 1-1 | SERPINA1 | A1AT1_MOUSE | 1 | 357 | −1.2 | 2.0 10−2 | −1.2 | 4.7 10−2 |
| Alpha-1-antitrypsin 1-2 | SERPINA1 | A1AT2_MOUSE | 1 | 683 | −1.5 | 2.0 10−3 | −1.4 | 2.4 10−2 |
| Alpha-2-HS-glycoprotein (Fetuin A) | AHSG | FETUA_MOUSE | 2 | 713, 849 | −1.2 | 8.4 10−4 | = | - |
| Alpha-2-macroglobulin | A2M | A2M_MOUSE | 3 | 996, 1009, 1010 | = | / | −1.5 | 5.4 10−4 |
| Antithrombin-III | SERPINC1 | ANT3_MOUSE | 3 | 1695, 1697, 648 | +1.2 | 1.2 10−3 | +1.1 | 5.2 10−3 |
| Apolipoprotein A-I | APOA1 | APOA1_MOUSE | 1 | 1335 | = | / | −1.3 | 6.0 10−4 |
| Apolipoprotein E | APOE | APOE_MOUSE | 3 | 1080, 1099, 1106 | −1.2 | 6.6 10−3 | = | / |
| Apolipoprotein H (Beta-2-glycoprotein I) | APOH | APOH_MOUSE | 1 | 626 | +1.2 | 4.4 10−3 | +1.2 | 8.2 10−3 |
| Clusterin | CLU | CLUS_MOUSE | 7 | 938, 1015, 1036, 1042, 1096, 1669, 1670 |
+1.3 | 2.8 10−8 | +1.2 | 5.7 10−7 |
| −1.2 | 1.2 10−3 | −1.2 | 2.2 10−2 | |||||
| Coagulation factor X | F10 | FA10_MOUSE | 1 | 778 | −1.2 | 2.2 10−3 | = | / |
| Complement C1r-A subcomponent | C1R | C1RA_MOUSE | 2 | 475, 477 | +1.2 | 2.3 10−3 | +1.3 | 6.3 10−5 |
| Complement C1s-A subcomponent | C1S | CS1A_MOUSE | 1 | 476 | +1.1 | 5 10−3 | = | / |
| Complement C3 | C3 | CO3_MOUSE | 4 | 579, 595, 1205, 1677 | −1.3 | 2.9 10−3 | −1.2 | 2.3 10−3 |
| Complement C4-B | C4A | CO4B_MOUSE | 1 | 1594 | −1.3 | 1.7 10−2 | = | / |
| Complement component 7 | C7 | CO7_HUMAN | 5 | 400, 401, 407, 408, 412 | −1.3 | 7.5 10−4 | −1.4 | 2.9 10−2 |
| Complement factor H | CFH | CFAH_MOUSE | 1 | 164 | −1.1 | 4.5 10−3 | = | / |
| Complement factor I | CFI | CFAI_MOUSE | 1 | 1057 | +1.1 | 1.5 10−3 | +1.2 | 2.0 10−3 |
| Fetuin B | FETUB | FETUB_MOUSE | 2 | 673, 676 | +1.2 | 1.7 10−5 | +1.1 | 2.4 10−3 |
| Group-specific component (Vitamin D binding protein) | GC | VTDB_MOUSE | 2 | 667, 678 | +1.3 | 7.4 10−4 | +1.3 | 4.3 10−3 |
| Haptoglobin | HP | HPT_MOUSE | 2 | 931, 942 | +7.1 | 2.1 10−7 | +2.5 | 4.6 10−2 |
| Hemopexin | HPX | HEMO_MOUSE | 9 | 499, 509, 511, 512, 519, 541, 578, 1681, 1692 |
−1.3 | 2.3 10−3 | +1.2 | 6.9 10−3 |
| +1.3 | 4.5 10−5 | |||||||
| Ig mu chain C region secreted form | Ighm | IGHM_MOUSE | 2 | 319, 358 | −1.3 | 7.9 10−3 | −1.1 | 2.0 10−2 |
| Inter alpha-trypsin inhibitor, heavy chain 4, isoform CRA_b | ITIH4 | A6X935_MOUSE | 1 | 345 | +1.1 | 1.0 10−2 | +1.1 | 4.4 10−2 |
| Kininogen-1 | KNG1 | KNG1_MOUSE | 1 | 569 | +1.3 | 1.2 10−4 | = | / |
| Murinoglobulin-1 | Mug1 | MUG1_MOUSE | 1 | 627 | +1.1 | 2.5 10−2 | +1.1 | 2.8 10−3 |
| Novel protein similar to odorant binding protein Ia Obp1a | Gm5938 | A2AEN9_MOUSE | 1 | 1653 | +1.5 | 1.1 10−2 | = | / |
| Odorant binding protein Ia | Obp1a | P97336_MOUSE | 1 | 1632 | +1.6 | 4.2 10-3 | = | / |
| Pantothenate kinase 4 | PANK4 | PANK4_MOUSE | 1 | 1129 | +1.9 | 2.9 10-11 | +1.6 | 3.6 10−7 |
| Peroxiredoxin-2 | PRDX2 | PRDX2_MOUSE | 1 | 1413 | = | / | −1.3 | 1.6 10−2 |
| Glycosylphosphatidylinositol specific phospholipase D1 | GPLD1 | PHLD_MOUSE | 2 | 355, 520 | −1.3 | 8.0 10-4 | −1.2 | 6.4 10−3 |
| Plasminogen | PLG | PLMN_MOUSE | 2 | 309, 415 | −1.2 | 1.1 10-3 | −1.7 | 4.2 10−2 |
| +1.6 | 7.9 10-3 | +1.6 | 2.6 10−2 | |||||
| Serine protease inhibitor A3K | Serpina3k | SPA3K_MOUSE | 12 | 54, 86, 87, 256, 257, 262, 531, 588, 599, 621, 1671, 1672 | −1.6 | 2.0 10-5 | −1.3 | 4.0 10−2 |
| Serum paraoxonase/ arylesterase 1 | PON1 | PON1_MOUSE | 1 | 803 | −1.2 | 5.0 10-3 | = | / |
| Zinc-alpha-2-glycoprotein | AZGP1 | ZA2G_MOUSE | 2 | 787, 790 | +1.1 | 3.1 10-2 | +1.1 | 4.1 10−4 |
1 Several differentially expressed spots were identified as same proteins; 2 Spot numbers were assigned by Progenesis SameSpots software according to their position in 2-DE gels; 3 Max Fold Changes associated with a p-value < 0.05. Fold changes and results of statistical tests (p- and q-values, Power) are comprehensively given in Supplemental Table S3. The different spots of Clusterin, Hemopexin and Plasminogen could display opposite trends of expression, which is why two different Max Fold Changes have been reported in the Table; 4 Max fold changes are given for irradiated samples compared to controls (sham-irradiated) samples; 5 Max fold changes are given for comparisons of irradiated samples (20, 40 and 80 Gy) (the highest dose versus the lowest dose).