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. 2014 Jun 26;2(3):303–322. doi: 10.3390/proteomes2030303

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Second dimension gel electrophoresis for samples exposed to three days of waterlogged soil. (a) Guaiacol staining of the second dimension modified SDS-PAGE after separation by IEF-PAGE in the first dimension. The pI of the guaiacol detected spots in the first dimension was indicated at the top of the gel; (b) CCB staining of the gel shown in (c); (c) Guaiacol staining of the second dimension hrCNE after separation by IEF-PAGE in the first dimension. The pI of the guaiacol detected spots in the first dimension is indicated at the top of the gel.