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. 2017 Feb 10;12(2):e0171850. doi: 10.1371/journal.pone.0171850

Fig 2. Complement factors are necessary for efficient neutrophil-mediated phagocytosis of apoptotic cells.

Fig 2

Neutrophils were co-incubated with apoptotic cells in the presence of 30% normal human serum, C1q-depleted or C3-deficient serum with or without reconstitution of the deficient sera with exogenous C1q or C3, respectively. Phagocytosis of apoptotic cells was assessed by flow cytometry. Data are shown as mean ± SEM of four independent experiments (A). Neutrophils were infected with L. major promastigotes (ratio of 1 to 10) for 4 h at 37°C. Surface expression of CD11b (B), CD35 (C), CD11c (D), CD14 (E), and CD44 (F) was assessed by flow cytometry. Data are shown as mean ± SEM of three to seven independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ns = not significant.