Figure 3. Structures of TCPM in ΔtcpS, ΔtcpB, ΔtcpD and ΔtcpR cells.
(a, b, c, d, e) Central slices of sub-tomogram averages of TCPM basal bodies from ΔtcpS, ΔtcpB, ΔtcpD, ΔtcpR and wild-type cells, respectively. Arrows in (d) indicate the cytoplasmic disc-like density. (f, g, h, i) Differences in the ΔtcpS, ΔtcpB, ΔtcpD and ΔtcpR structures compared to the wild-type, respectively (addition and omission of densities are shown by red or yellow color, respectively, with weak to strong intensities corresponding to density differences from one to three standard deviations, overlaid on the wild-type sub-tomogram average). (j) Differences in the ΔtcpS structure compared to the ΔtcpD structure (difference maps overlaid on the ΔtcpD sub-tomogram average). Arrows indicate a larger decrease of the mid-periplasmic ring density caused by ΔtcpS than by ΔtcpD. Scale bars, 10 nm.