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. 2017 Feb 10;12(2):e0171854. doi: 10.1371/journal.pone.0171854

Fig 1. AtRGS1 functions in an early step in ROS production.

Fig 1

(A) flg22-induced, FLS2-dependent ROS production is greatly attenuated in the rgs1 null mutant. ROS, reported as Relative Luminescence Units (RLU) in leaf disks treated with flg22 (1 μM) from 5-week-old seedlings was measured over time (S1 Fig) as described in Materials and Methods. The graph shows the maximum relative light units (Max RLU) taken at the peak of ROS production and is plotted for all genotypes. Error bars are SEM and sample size n = 6–15. *, P<0.05, *** P<0.001 by Student's t-test compared to wild type (wt). (B) flg22-induced Ca2+ signal requires AtRGS1. Fluorescence intensity changes of R-GECO1 in ~50 regions of interests in wild type, rgs1-2 and bak1-4 plants. Fractional fluorescence changes (ΔF/F) for R-GECO1 were calculated from background corrected intensity values of R-GECO1 as (F − F0)/F0, where F0 represents the average fluorescence intensity of the baseline of a measurement of each genotype. Error bars are standard deviations.