Fig. 4. Loaded in vitro motility assays for the three mutant proteins compared to WT controls.

The percent time mobile (11) is a measure of the relative effectiveness of the load molecule utrophin to overcome the F_ensemble of the sS1 on the surface (see Materials and Methods) (11). The effect of utrophin concentration on the velocity of actin (left) and RTFs (in 10⁻⁵ M Ca²⁺) (right) is shown. For WT and each mutant protein, ≥5 independent protein preparations and ≥5 separate sets of curves were combined.