Figure 5. V61C:V61C' linkage is a marker of dimer-dimer interaction.
(A) BNP analysis of V61C:V61C' linkage revealed no dimers, and a high proportion of 4x and >10x species. In contrast, H164C:H164C’ linkage revealed a ladder of linked species. Data are representative of nine biological replicates. (B) Densitometry of V61C:V61C' BNP linkage in Bak oligomers shows negligible dimers but reproducible linked species at 4x, 6x, 8x, and 10x, and a large population of species > 10x. Densitometry data (n = 9) were normalised to the area under the curve after alignment as described in Materials and Methods. To correct for small variations in electrophoretic migration, we employed a noise reduction algorithm in which the 4x peak (0) and a minima at ~720 kDa (1) were aligned between replicates.