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. 2016 Aug 10;7(35):56060–56070. doi: 10.18632/oncotarget.11180

Figure 1. Expression of C5a protein in vitro and in vivo after adenoviral gene transfer.

Figure 1

A., Fluorescence images of HEK293 cells after transfection with phosphate buffered saline (PBS) or different multiplicities of infection (MOI) of adenovirus C5a (Ad-C5a) for 24 hr. B., Migration assay of chemotaxis of recombinant C5a to macrophages. HEK293 cells were transfected with Ad-GFP and different MOI (1:1, 10:1 and 100:1) of Ad-C5a. At 24 hr, the supernatants underwent trans-well assay. C., Quantitative analysis of trans-well assay. Data are mean ± SEM from 5 separate fields in each sample from 3 independent experiments. **P < 0.01. D., Detection of recombinant mouse C5a protein in ApoE−/− mouse plasma (n = 5). *P < 0.05 and **P < 0.01 vs day 0.