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. 2016 Aug 12;7(35):56107–56119. doi: 10.18632/oncotarget.11271

Figure 2. Overexpression of HBx and HBc genes suppresses MICA expression in hepatoma cells.

Figure 2

A. pEGFP-N1, pEGFP-HBx, pEGFP-HBc, pEGFP-HBs or pEGFP-HBp was transfected into HepG2 cells, and 48 h later MICA and MICB expression levels were analyzed by RT (reverse transcription) -PCR. B. HepG2 cells were transfected with a reporter plasmid containing the MICA or MICB promoter together with pEGFP-N1, pEGFP-HBx, pEGFP-HBc, pEGFP-HBs or pEGFP-HBp for 36 h. Renilla luciferase activity was normalized to firefly luciferase activity. C. HepG2, PLC/PRF/5, H7402 and HL7702 cells were transfected with pEGFP-N1, pEGFP-HBx, pEGFP-HBc, pEGFP-HBs or pEGFP-HBp for 48 h, and then MICA/B expression was analyzed by flow cytometry. D. Western blot analysis of MICA expression in HepG2 cells after transfection with pEGFP-N1, pEGFP-HBx, pEGFP-HBc, pEGFP-HBs or pEGFP-HBp for 48 h. The densitometry analysis of MICA expression normalized to GFP. **P < 0.01; *P < 0.05, compared with HepG2-N1 (paired t-test).