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. 2016 Aug 2;7(35):56219–56232. doi: 10.18632/oncotarget.11019

Figure 2. CD3CAR NK-92 cells eliminate CD3-expressing T-ALL cell lines in vitro.

Figure 2

A. Co-cultures with CD3CAR NK-92 performed at an effector:target (E:T) ratio of 2:1. T-lymphoblast cell line Jurkat (90% CD3+) co-cultured for 6 hours. Sorted CD3+ CCRF-CEM (CCRF-CEMCD3+) co-cultured for 24 hours. Negative control, CD3- non-Hodgkin's Lymphoma cell line KARPAS 299 co-cultured for 24 hours. Target populations quantified with flow cytometry using CD56 and CD3 to distinguish NK and target cell populations. CD4 used to determine KARPAS population. Populations encircled to highlight lysis amount. B. Co-cultures with CD3CAR NK-92 performed at an effector:target (E:T) ratio of 5:1. Experimental conditions identical as 2:1. C. Graphical summary of CD3CAR NK-92 in vitro assays against T-ALL cell lines. Each bar represents the average % cell lysis for duplicate samples; N = 4 experiments for Jurkat and CCRF-CEMCD3+ and N = 2 experiments for KARPAS. D. Absolute cell counts of CCRF-CEMCD3+ and Jurkat cultures with CD3CAR NK-92 cells at an E:T ratios of 5:1. Control and CD3CAR treatment samples are labeled in red and blue respectively with effector and target cell counts performed via FACS analysis from Kaluza flow cytometry software (Beckman Coulter).

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