Figure 4. CD3NK-CAR NK-92 dose-dependent lysis of malignant cells.

A. Flow cytometry schemes showing dose-dependent CD3CAR NK-92 co-cultures with CCRF-CEM^CD3+ and peripheral blood derived (PB) T-cells. E:T ratios were decreased to a lower bound of 0.25:1 (25,000 effector cells to 100,000 target cells) and encircled populations represent lysis effects of interest. Co-cultures were conducted over 24 hours. B. Dose-dependent flow analysis of CD3CAR NK-92 cells with Jurkat cell line. Co-cultures were conducted for 6 hours and populations analyzed. C. Dose-dependent co-cultures of CD3CAR NK-92 cells with PT4 (unclassified PTCL) primary patient samples obtained from bone-marrow aspirate. D. Bar graphs summarizing the dosage relationships between CD3CAR NK-92 dose and target cell depletion. Bars represent the average of duplicate samples with CCRF-CEM^CD3+ (N = 4), Jurkat (N = 4), PB T-cell (N = 2), and PT4 (N = 4).