Skip to main content
. 2016 Jul 28;7(35):56355–56370. doi: 10.18632/oncotarget.10889

Figure 3. In vivo and in vitro expression of Gas6 in human and murine macrophages.

Figure 3

(A) CD68 (red) and Gas6 (green) double staining in human colorectal cancer tissue sample showing co-expression (orange) of the two antigens in tumor infiltrating macrophages (40 × magnification, scale bar = 50 μm). (B) Staining for CD68 as a macrophage marker in human colorectal cancer tissue sample showing infiltrating macrophages (brown; 10 × magnification, scale bar = 200 μm). (C) Sequential staining in the same tissue area for Gas6 in human colorectal cancer tissue sample showing Gas6 expression in tumor infiltrating cells. Gas6 co-localizes to CD68 macrophages in the previous section (brown; 10 × magnification, scale bar = 200 μm). (D–F) RT-PCR analysis showing a reduced Gas6 expression in murine macrophages differentiated to a M1 (LPS) phenotype and an increased Gas6 expression in a M2 (M-CSF) phenotype (n = 3, P < 0.01). (PM = peritoneal macrophages; BMDM = Bone marrow derived macrophages).