Skip to main content
. 2016 Jul 28;7(35):56355–56370. doi: 10.18632/oncotarget.10889

Figure 4. Gas6 induces proliferation, colony-, sphere-formation and invasion in human colorectal cancer cell lines in vitro.

Figure 4

(A) Human recombinant Gas6 (rhGas6) dose-dependently induces proliferation of human colorectal cancer cells (HCT116) (n = 6; P < 0.05). (B) Colorectal cancer cells (HCT116) treated with 100 ng/ml recombinant human Gas6 form significantly more spheres after 10 days compared to control treated cells (n = 3; P < 0.05). (C) Colorectal cancer cells (HCT116) treated with 100 ng/ml recombinant human Gas6 form significantly more colonies after 10 days compared to control treated cells (n = 3; P < 0.05). (D) Invasion of human colorectal cancer cells (HCT116) is increased after 24 hours treatment with recombinant human Gas6 (rhGas6) in vitro (n = 9; P < 0.05). 20% FCS was used as positive control (n = 9; P < 0.05). (E) siRNA knockdown of the TAM receptors in human colorectal cancer cells (HCT116) lead to an significantly decreased proliferation. The most distinct effect was observed for Tyro3 > Mer > Axl (n = 6; P < 0.05).