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. 2016 Jul 28;7(35):56355–56370. doi: 10.18632/oncotarget.10889

Figure 6. Macrophages upregulate Gas6 under 5-FU treatment and mediate a chemoprotective effect.

Figure 6

(A) RT-PCR analysis revealing no upregulation of Gas6 in human colorectal cancer cells (HCT116) after 24 hours 5-FU treatment (n = 3; P = NS). (B) RT-PCR analysis revealing significant upregulation of Gas6 in murine macrophages (J774A.1) after 24 hours 5-FU treatment (n = 3; P < .05). (C) 5-Fluorouracil dose-dependently reduces proliferation of human colorectal cancer cells (HCT116) in vitro after 48 hours treatment (n = 6; P < .05). (D) The antiproliferative effect of 5-Fluorouracil was partially antagonized by Gas6 in colorectal cancer cells (HCT116) treated with recombinant human Gas6 (100 ng/ml) and 5-Fluorouracil (5 μM) in vitro (n = 6; P < .05). (F) Co-cultures of murine colorectal cancer cells (CT26) with murine macrophages (J774A.1) reveal a chemoprotective effect of the macrophages likely mediated by Gas6 (n = 3, P < .05).