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. 2016 Jul 28;7(35):56422–56430. doi: 10.18632/oncotarget.10896

Figure 1. Repression of PML/RARα by c-Myc is miRNA dependent.

Figure 1

(A) QRT-PCR analysis of c-myc and PML/RARα mRNA changes at 24 hr following siRNA mediated silencing of c-myc with and without siRNA-mediated silencing of Dicer1. The data were normalized to nontarget siRNA (siNC) transfection. (B) Western blot showing c-myc and PML/RARα protein expression 48 h after transfection of the indicated siRNAs. (C) MicroRNA RT-PCR showing expression of let-7a, 7c and 7g in response to siRNA against c-myc alone or together with siRNA against Dicer1. siNC was used as a control. Bar graph was represented as mean ± SD of three independent experiments performed in triplicates (n = 3). ** indicated P < 0.01 and *** indicated P < 0.001. Representative blot was shown from three independent experiments.