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. 2016 Jul 28;7(35):56422–56430. doi: 10.18632/oncotarget.10896

Figure 4. C-Myc suppressed PML/RARα by let-7 miRNAs via 3′UTR.

Figure 4

(A) Luciferase activity in HEK293T cells cotransfected with PML/RARα3′UTR reporter construct and let-7 mimics (7a, 7c and 7g respectively) or scrambled microRNA control (miNC). (B) QRT-PCR analysis of c-myc and PML/RARα expression 48 hr after transfection of NB4 cells with let-7 mimics (let-7a, 7c and 7g respectively) and scrambled microRNA control (miNC). (C) Western blot analysis for c-myc and PML/RARα 48 hr after transfection of indicated mimics. (D) Efficiency of indicated let-7 ectopic expression, measured by qRT-PCR analysis. (E) Luciferase activity in HEK293T cells cotransfected with PML/RARα3′UTR reporter construct and siRNA against c-myc or nontarget control (siNC). Bar graph was represented as mean ± SD of three independent experiments performed in triplicates (n = 3). * indicated P < 0.05, ** indicated P < 0.01 and *** indicated P < 0.001. Representative blot was shown from three independent experiments.