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. 2016 Jul 18;7(35):56540–56557. doi: 10.18632/oncotarget.10645

Figure 7. XIAP RING domain promoted miR-4295 host gene VTI1A transcription in human bladder epithelial cells.

Figure 7

A. and B. The cell extracts were subjected to Western Blot to determine expression levels of VTI1A protein (A) and mRNA (B) in UROtsa(Nonsense) vs. UROtsa(shXIAP) cells (A); or UROtsa(Vector) vs. UROtsa(HA-ΔBIR) and UROtsa(HA-ΔRING) cells as indicated. C. and D. VTI1A promoter-driven luciferase reporter was transfected together with TK reporter into UROtsa(Nonsense) vs. UROtsa(shXIAP) cells (C), or UROtsa(Vector) vs. UROtsa(HA-ΔBIR) and UROtsa(HA-ΔRING) cells (D). Twenty-four hours post transfection, the transfectants were extracted to evaluate the luciferase activity, with TK used as internal control. The results were presented as luciferase activity relative to vector control transfectant. Each bar indicates mean±SD from three independent experiments. The symbol (*) shows a significant difference (P<0.05).