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. 2016 Jul 18;7(35):56558–56573. doi: 10.18632/oncotarget.10646

Figure 3. PXR transcriptional activity marks chemoresistant CSCs.

Figure 3

a. Flow cytometry profiles of EIF1α-eGFP and CYP3A4-eGFP infected T84 cells and regions used for cell-sorting. NT= Non infected, Lo=GFP low, Br=GFP bright. b. RT-qPCR analyses of PXR, PXR target and CSC marker gene mRNA expression in Br and Lo populations compared to EIF1α-GFP low cells (F.I., Fold Induction). Data are expressed as mean ± SEM (n=3). c. Percentage of surviving cells, 72 hours after exposure of sorted cells to the indicated dilutions of Firi (1X=50μM 5-FU + 500nM SN38). Data are expressed as mean ± SEM (n=4). d. Percentage of sphere-forming cells for Br and Lo populations. a,b,c: #: p<0.05 compared to Lo cells for each GFP construct, $: p<0.05 compared to EIF1α-GFP Br cells. e. Representative flow cytometry profiles of EIF1α-eGFP- and CYP3A4-eGFP infected cells before (2D NT) or after 72h of Firi (50μM 5-FU + 500nM SN38) treatment followed by 2 days of recovery without treatment (2D FIRI) or maintained as colonospheres (Sphe). The fold increase in GFP Br percentage induced by Firi (2D FIRI/2D NT) or colonosphere conditions (Sphe/2D NT) are indicated for each GFP construct as mean ± SEM (n>3). *, p<0.05; **, p<0.005.