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. 2016 Aug 4;7(35):56699–56712. doi: 10.18632/oncotarget.11057

Figure 1. Inhibition of YAP expression or activity in 5F31 is associated with cellular quiescence.

Figure 1

(A, B) Analysis of cell cycle distribution (G0-G1, S and G2-M phases) and percentage of G0 resting cells in 5F31 cells incubated in the presence and absence (control: Ctrl) of the YAP inhibitor Verteporfin (VP). Cells were treated by 10 μM VP for 48 hours and processed by flow cytometry for G0-G1, S, G2-M distribution and quantification of G0 phase cells using Ki-67 labelling. (C) Cell count after 48 hour treatment by 10 μM VP. (D, E) Flow cytometry analysis of cell cycle distribution (G0-G1, S and G2-M phases) and percentage of G0 quiescent cells in YAP-silenced vs control 5F31 cells. Cells were treated for 48 hours by 30 nM YAP siRNA or nontargeting siRNA (Ctrl cells). (F) Cell growth of YAP-silenced vs control 5F31 cells after 48 hour treatment by siRNA. All data are from 3 replicates.