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. 2016 Jul 22;7(35):56767–56780. doi: 10.18632/oncotarget.10790

Figure 3. HDAC3 activity was downregulated during delphinidin-induced apoptosis in LNCaP cells.

Figure 3

A. Delphinidin reduces total HDAC activity. Prostate cancer cells were treated with various concentrations of delphinidin (50, 100, and 150 μM) for 24 h. Non-treated cells were used as controls. Statistical significance was determined using Student's t-test; *P < 0.01 versus controls. B. Delphinidin inhibits HDAC3 activity. HDAC activity assays were performed using a colorimetric HDAC activity assay kit. The data are expressed as mean ± SD for triplicate measurements. Statistical significance was determined using Student's t-test; *P < 0.05 versus non-treated controls. C. HDAC3 expression was reduced by delphinidin treatment. LNCaP cells were treated with delphinidin. Cell lysates were processed for western blot analysis using the indicated antibodies. D. The viability of LNCaP cells was decreased by HDAC inhibitors and HDAC3 siRNA. LNCaP cells were treated with delphinidin and TSA (MS-275 or HDAC3 siRNA) for 12 h. The cell viability was measured using the MTT assay. All data are expressed as the mean ± SD for triplicates. Statistical significance was determined using Student's t-test; *P < 0.01 versus LNCaP cells that were not treated with delphinidin.