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. 2016 Jul 23;7(35):56811–56825. doi: 10.18632/oncotarget.10796

Figure 2. Induction of apoptosis by MNKI-8e, Ara-C or their combination in MV4-11 cells.

Figure 2

A. MV4-11 cells were exposed to 5 μM MNKI-8e, 0.1 μM Ara-C or their combination for 24 h and analyzed by annexin V/PI staining. The percentage of cells undergoing apoptosis was defined as a sum of early (annexin V+/PI-) and late (annexin V+/PI+) apoptotic cells. B. Caspases 3/7 activation assay of MV4-11 cells following 24 h treatment with 5 μM MNKI-8e, 0.5 μM Ara-C or their combination in the presence or absence of Z-VAD-FMK (20 μM). C. Induction of apoptosis by MNKI-8e and/or Ara-C in the presence or absence of Z-VAD-FMK by annexin V/PI staining. DMSO diluent was used as control in each experiment. Vertical bars represent the mean ± SD of three independent experiments. Data were analyzed by the Student's t-test *p ≤ 0.05 or **p ≤ 0.01.