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. 2016 Aug 1;7(35):57086–57098. doi: 10.18632/oncotarget.10977

Figure 3. STAT4 modulates the levels of p66Shc in B cells.

Figure 3

A. qRT-PCR analysis of STAT4 and p66shc mRNA in EBV-B cells transiently transfected with either empty vector (Ctr) or an expression construct encoding STAT4 (STAT4). B. Immunoblot analysis of p46Shc, p52Shc and p66Shc 48 h after transfection with the STAT4-encoding construct in EBV-B cells. The histogram shows the quantification of the p66Shc immunoreactive band (n≥3). C. EBV-B cells were transfected with esiRNA targeting STAT4 and the levels of p66shc and STAT4 were measured by qRT-PCR. The relative abundance of the gene transcripts was determined on triplicate samples from at least 3 independent experiments using the ΔΔCt method and is expressed as the normalized fold expression (mean±SD). D. Immunoblot analysis of p46Shc, p52Shc and p66Shc 48 h after transfection of EBV-B cells with esiRNA targeting STAT4 (n≥3). Filters were reprobed for actin as loading control. The histogram shows the quantification of the p66Shc immunoreactive band. **P≤0.01; and *P≤0.05.