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. 2016 Aug 9;7(35):57213–57227. doi: 10.18632/oncotarget.11127

Figure 6. Involvement of sirtuin 1 (SIRT1) in transforming growth factor (TGF)-β1-induced epithelial-mesenchymal transition (EMT) inhibited by celecoxib and sulindac.

Figure 6

A. and B. Effect of SIRT1 inhibition on TGF-β1-induced EMT. Cells were transfected with SIRT1 siRNA or treated with the SIRT1 inhibitor EX-527, and then further incubated in the presence of celecoxib or sulindac for 24 h. The cell lysates of each group were prepared and probed for EMT hallmarks by western blot. C and D. Effect of SIRT1 activation on TGF-β1-induced EMT. Cells were transfected with Ad-lacZ or Ad-SIRT1, or treated with the SIRT1 activator SRT-1720, and then further incubated in the presence of celecoxib or sulindac for 24 h. The cell lysates were routinely prepared, and alterations in EMT hallmarks were determined by western blot.