Skip to main content
. 2016 Aug 9;7(35):57239–57253. doi: 10.18632/oncotarget.11136

Figure 4. miRNA-mediated gene regulation events in tamoxifen-resistant cell lines and functional evaluation of their importance in tamoxifen resistance.

Figure 4

A. Consistent downregulation of estrogen receptor-alpha and progesterone receptor by several miRNAs exhibiting altered expression in TamR1, TamR4 or TamR8 relative to MCF-7/S0.5, as indicated using node colors. B. FOXM1 expression is regulated by a number of miRNAs in TamR4. Suppressed expression of FOXM1 positively correlated with the expression of its transcriptional targets, including miR-135a, let-7f and miR-365. C. YWHAG and YWHAZ appeared to be under miRNA regulation in TamR8. Color and intensity of the nodes correspond to the extent of fold-changes observed in the relevant comparison. D. Growth of MCF-7/S0.5 and TamR cell lines following treatment with the selective P53-SNAIL binding inhibitor GN25 (3μM) or its solvent, DMSO (Control), or E. the selective SKF inhibitor PP2 (2.5 μM) or its solvent, ethanol (Control) in medium with or without tamoxifen (1μM). Cells were also treated with combined GN25 and PP2. Cell growth was determined at 72 hrs using a colorimetric assay. F. Expression of SNAI2 in MCF7 or TamR cell lines following SNAI2-specific or control siRNA transfection as measured by qRT-PCR. G. Growth of cells in tamoxifen-containing medium measured 4 days after siRNA transfection, * p<0.01, ** p<0.001.