Figure 5. ROC curve analysis to assess the ability of miRNAs to predict outcome of cohorts of ER+ breast cancer patients receiving tamoxifen monotherapy.

Selected miRNAs that exhibited altered expression in TamRs vs. MCF-7/S0.5 cell lines were also predictive of recurrence following tamoxifen treatment. The 2-miR classifier, miR-190b and miR-516a-5p, was fit on cohorts 1 and 2 (Fit cohort) and then applied to both cohorts independently. Training performance is observed when Fit cohort and test cohorts are the same (dotted lines), while independent test set performance is observed when they differ (solid lines).