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. 2016 Aug 9;7(35):57254–57267. doi: 10.18632/oncotarget.11138

Figure 6. ARID1A-EpCAM is involved in oncogenicity and stemness of HNSCC cells.

Figure 6

A-G. OECM1 and FaDu cells. (A, B) Ectopic ARID1A expression, (C, D) Knockdown of ARID1A expression. (A, C) Western blot analysis. (B, D) qRT-PCR analysis. An inverse relationship between the expression level of ARID1A and EpCAM should be noted. (E) Ectopic miR-31 expression. This results in ARID1A upregulation and EpCAM downregulation. (F) Treatment with siEpCAM oligonucleotide. This downregulates EpCAM protein expression. (G) Upper, OECM1 cell; Lower, FaDu cell. Analysis of proliferation (Lt), migration (Middle) and invasion (Rt). H. FaDu cell. Knockdown of EpCAM decreases the ALDH1+ (Lt) and CD44+ (Rt) cell populations. The numbers below the Western blot pictures are the normalized values.