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. 2017 Feb 8;96(2):295–303. doi: 10.4269/ajtmh.16-0642

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Figure 1. — C4b deposition is inhibited in blood recovered from the Triatoma infestans anterior midgut. Plates were sensitized with IgM and C4b deposition (a direct reflection of C4 activation) was measured in blood recovered from the T. infestans anterior midgut (TMB) immediately after feeding. This C4b deposit was compared with chicken normal serum (CNS) obtained from the same bird used to feed the insects. CNS plus ethylenediaminetetraacetic acid (CNS + EDTA) and TMB plus EDTA (TMB + EDTA) were positive inhibition controls. The results correspond to the mean of triplicates and were analyzed by one-way analysis of variance (P < 0.0001) using the GraphPad Prism Program version 6.0c.