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. 2017 Feb 8;96(2):295–303. doi: 10.4269/ajtmh.16-0642

Figure 5.

Figure 5.

Human C1 binds to the TiCRT recombinant S domain. (A) rTiS was incubated with human C1, followed by a goat anti-C1 antibody and a rabbit alkaline phosphatase–conjugated anti-goat IgG antibody. (B) As a negative control, rTiS was incubated with 3% phosphate-buffered saline (PBS)/skimmed milk, followed by goat anti-C1 antibody and a rabbit alkaline phosphatase–conjugated anti-goat IgG antibody. (C) As a positive control, C1 was detected with an anti-C1 antibody, followed by a rabbit alkaline phosphatase–conjugated anti-goat IgG antibody.