Protein extracts were generated from ET of control (n=34), CRSsNP (n=83) and CRSwNP (n=45) and from NPs (n=60). Expression of ECP, IL-5, IL-13, IFN-γ and IL-17A proteins in tissue homogenates was measured using ELISA and Luminex. The protein concentrations were normalized to the concentration of total protein. Dotted line indicates the threshold based on the 95th percentile expression in control ET (ECP: 131.5 ng/mg, IL-5: 0.02 pg/mg (detectable), IL-13: 5.5 pg/mg, IFN-γ: 8.1 pg/mg, IL-17A: 0.02 pg/mg (detectable)). In order to display undetectable data, we plotted 0 as 0.01 in the log scaled figures. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001, by one-way ANOVA.