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. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: Anal Biochem. 2017 Jan 11;521:55–58. doi: 10.1016/j.ab.2017.01.008

Fig. 1. The use of TEV-cleaved SrtA 5M to label EGFR containing varying N-terminal glycines.

Fig. 1

A. Comparing sortase activity of 6x-His SrtA 5M and TEV-cleaved SrtA 5M. B. Comparison of labeling of Gly-EGFR, Gly2-EGFR and Gly4-EGFR using an LPETG peptide substrate containing the TAMRA fluorophore.