ltgA+ ltgD+ double complement, ΔltgAΔltgD double mutant, and ΔltgAΔltgDΔampG triple mutant Gc were exposed to adherent, IL-8-treated neutrophils. For ΔltgAΔltgD treated with monomer, Gc infected neutrophils were exposed to 1 µg/mL monomer for the duration of the infection. *P<0.05 for ΔltgAΔltgD with monomer compared to double complement and for ΔltgAΔltgDΔampG compared to double complement; two-tailed t-test, n = 3 independent experiments.