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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Cell Microbiol. 2016 Nov 3;19(3):10.1111/cmi.12662. doi: 10.1111/cmi.12662

Figure 4. ΔltgAΔltgD sensitivity to PG digestion occurs independently of PG acetylation.

Figure 4

A. ΔltgAΔltgD double mutant, ltgA+ltgD+ double complement, ΔpacA, ΔltgAΔltgDΔpacA, and pacA+ complement in ΔltgAΔltgDΔpacA were exposed to 100 µg/mL human lysozyme for 90 min. Gc survival is expressed as in Fig. 3A. *P<0.05; two-tailed t-test, n = 3 to 6 biological replicates.

B. ΔltgAΔltgD double mutant and ltgA+ltgD+ double complement Gc were exposed to increasing concentrations of mutanolysin for 3 hr. Gc survival is expressed as in Fig. 3A. *P<0.05; two-tailed t-test, n = 3 biological replicates.