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. 2017 Feb 1;26(2):243–257. doi: 10.3727/096368916X693031

Figure 4.

Figure 4.

MicroRNA 133b (miR-133b)-enriched exosomes enhance the release of exosomes from primary cultured oxygen-and glucose-deprived (OGD) astrocytes. MSC exosomes significantly increased the exosomes released by OGD astrocytes, and there was no obvious difference on the release from OGD astrocytes, after treatment with harvested from naive MSCs (Ex-Naive) and two control exosomes (A). Compared with the exosome Ex-Naive treatment, exosomes released by OGD astrocytes treated with miR-133b-overexpressing MSCs (Ex-miR-133b+) were significantly increased (A) and significantly decreased (B) by treatment with Ex-miR-133b−, respectively. Correlated with the miR-133b levels in the multipotent mesenchymal stromal cells (MSCs) (Table 1), the number of exosomes released by MSCs has no difference between naive MSCs and the two control MSCs (miR-CON+MSCs and miR-CON−MSCs), but the exosomal release was significantly increased from the miR-133b+MSCs and decreased from the miR-133b−MSCs, compared with that from naive MSCs (B). NivM, naive MSCs; NivA, naive astrocytes; NON, nontreated OGD astrocytes; MSC, OGD astrocytes treated with naive MSC exosomes; CON−, miR-CON−MSC or OGD astrocytes treated with miR-CON−MSC exosomes; 133b−, miR-133b−MSC or OGD astrocytes treated with miR-133b−MSC exosomes; CON+, miR-CON+MSC or OGD astrocytes treated with miR-CON+MSC exosomes; 133b+, miR-133b+MSC or OGD astrocytes treated with miR-133b+MSC exosomes. ∗p < 0.05, mean ± SD, n = 3/group.