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. 2017 Feb 1;26(2):243–257. doi: 10.3727/096368916X693031

Figure 5.

Figure 5.

Exosomes harvested from oxygen- and glucose-deprived (OGD) astrocytes after treatment with miR-133b-overexpressing MSCs (Ex-miR-133b+) increase neurite outgrowth. (A) The morphology of cultured neurons in this assay. Exosomes harvested from OGD astrocytes significantly increased the neurite number and total neurite length per neuron compared with the non-exosome-treated primary cultured cortical neurons. The neurite number and total neurite length substantially increased after the neurons were treated with exosomes harvested from OGD astrocytes that were pretreated by the MSCs infected with blank vector (Ex-Con) and Ex-miR-133b+, and Ex-miR-133b+-pretreated OGD astrocytic exosomes have the enhanced effect compared with the Ex-Con pretreatment (p < 0.05, respectively; B–D). Con, non-exosome-treated neurons; OGD, neurons treated with exosomes harvested from OGD astrocytes; Con+, neurons treated with exosomes harvested from OGD astrocytes pretreated with Ex-Con; 133b+, neurons treated with exosomes harvested from OGD astrocytes pretreated with Ex-miR-133b+. ∗p < 0.05, mean ± standard deviation (SD), n = 3/group. Scale bar: 50 μm.