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. 2017 Feb 1;26(2):243–257. doi: 10.3727/096368916X693031

Figure 6.

Figure 6.

MicroRNA 133b (miR-133b)-enriched exosomes downregulate the protein level of Rab9 effector protein with kelch motifs (RABEPK). Western blot data show that the MSCs infected with blank vector (Ex-Con) treatment significantly downregulated the RABEPK level increased in the ischemic boundary zone (IBZ) after middle cerebral artery occlusion (MCAO), and miR-133b-overexpressing MSC (Ex-miR-133b+) treatment further reduces the protein level (A, B) (p < 0.05, respectively). Double immunostaining shows the RABEPK largely expressed in the astrocytes (E–H). Ex-Con treatment significantly reduced the RABEPK level increase in the astrocytes subjected to oxygen and glucose deprivation (OGD), and Ex-miR-133b+ treatment further reduces the protein level (C, D) (p < 0.05, respectively). Sham, tissue from sham surgery rats; PBS, MCAO rats treated with phosphate-buffered saline (PBS); Naive, naive astrocytes; OGD, astrocytes subjected to OGD; CON+, MCAO rats or OGD astrocytes treated with Ex-Con; 133b+, MCAO rats or OGD astrocytes treated with Ex-miR-133b+. ∗p < 0.05, mean ± standard deviation (SD), n = 3/group. Scale bar: 50 μm.