Fig. 1.

Pre-exposure of neonate dermal fibroblasts to TNF augments their response to re-stimulation. a The experimental procedure. BJ fibroblasts received vehicle or 10 ng/mL TNFα for 24 h, and were then washed and rested for 24 h after collection of supernatants. Cells were then washed again and treated with vehicle or 10 ng/mL TNFα for a further 24 h before collection of supernatants (Collect sup.). Stim stimulation, W wash. b Fibroblast secretion of IL-6 in response to first or second doses of vehicle or TNFα. c Fibroblast secretion of IL-6 under the same conditions, with addition of 1 μg/mL adalimumab (Ad) either at the same time as the first TNFα stimulus (third column) or during the rest period between the first and second TNFα stimuli (sixth column). Mean ± SEM, n = 3–6. **p < 0.01; ***p < 0.005; n.s. not statistically significant